This measuring device allows researchers to detect protein-DNA binding interactions in less than 10 minutes, and determine binding constants within less than 1 hour. In a later phase, the application range will be extended to protein-protein interactions.
Instead of using a gel, as in a gel shift assay or electrophoretic mobility shift assay (EMSA), our technology relies on a nanopore to detect a mobility shift. As a consequence, our technology
- is fast: it takes less than 1 h per experiment instead of 2-3 days
- is label-free, eliminating the need for radio-active material or EtBr
- is easy to use: it requires pipetting buffer solution and different protein and DNA concentrations onto a chip.